I was one of the developers/knowledge engineers of the SpinPro™ Ultracentrifugation Expert System at Beckman Instruments, Inc. This was released in 1986, developed over about 2 years. This ran on an IBM PC (DOS)! This was a technical success, but not a commercial one. (The sales force was unfamiliar with promoting a software product, and which had little impact on their commissions vs. selling multi-thousand dollar equipment.) https://pubs.acs.org/doi/abs/10.1021/bk-1986-0306.ch023 (behind ACS paywall)
Our second Expert System was PepPro™, which designed procedures for the chemical synthesis of peptides (essentially very small proteins). This was completed and to be released in 1989, but Beckman discontinued their peptide synthesis instrument product line just two months before. This system was able to integrate end-user knowledge with the built-in domain knowledge. PepPro was recognized in the first AAAI Conference on Innovative Applications of Artificial Intelligence in 1989. https://www.aaai.org/Papers/IAAI/1989/IAAI89-010.pdf
Both of these were developed in Interlisp-D on Xerox 1108/1186 workstations, using an in-house expert system development environment, and deployed in Gold Hills Common Lisp for the PC.